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Fig. 3
Exposure to iAs causes steatosis in zebrafish larvae. (A) Representative bright-field images of 5-dpf Oil Red O (ORO)-stained control and iAs-exposed (1.0 mM from 4 to120 hpf) larvae. The area around the liver (outlined in black) is enlarged. (B) The percent of larvae with steatosis analyzed by ORO staining of 15 clutches, with an average of 20 larvae per treatment per clutch. The total number of larvae analyzed in each clutch is listed in Table S1. Statistical significance was determined by unpaired, 2-tailed Student's t-test (n=15 clutches, P<0.001, Table S1). Error bars correspond to mean±s.d.
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Full text @ Dis. Model. Mech.