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Fig. 4
Ventral floor plate is aberrant in rfx4 mutant embryos by 24 hpf. In rfx4uw8013/+ sibling embryos, shha is expressed in the cranial primordium (A) and in the spinal cord floor plate, adjacent to notochord (B). Cranial expression of shha is unchanged in rfx4uw8013 homozygotes (C), while its expression in the spinal cord floor plate is strongly reduced (D). E,F: Expression pattern of ptc1, a target of Shh signaling, resembles that of shha in normal siblings. G,H: ptc1 expression is reduced in mutant spinal cord. fp, floor plate; sc, spinal cord.
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