Fig. 2
Par2b deficiency prevents grossly abnormal skin morphology in hai1a morphants. (A–D) Wild-type or par2b−/− embryos were injected with the indicated MOs and imaged at 30 hpf. Images of control (A), hai1a morphant (B), par2b−/−:hai1a morphant (C), and par2b−/− embryos (D) are shown. Note the presence of cell clusters on hai1a morphants (B, arrowheads) and their absence on controls and Par2b-deficient hai1a morphants. Bar, 200 µm. (E) Percentage of embryos with cell clusters. 100 or more embryos were analyzed for each condition; mean ± SEM for three independent experiments is shown. Statistical analysis was performed using one-way ANOVA and Bonferroni posttest. The hai1a morphant was different from all other groups (P < 0.0001).