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Figure Caption
Fig. 6-S3
M2 muscarinic receptor is expressed at the venous pole.
(A–B) In situ hybridization for the muscarinic 2a cholinergic receptor (chrm2a) in wild-type embryos at 55 hpf (A) and 72hpf (B). (C) ISH for chrm2a at 72 hpf in Axin1-YFP+ embryo (Wnt inhibition started at 52 hpf). Arrowheads indicate expression at the venous pole. Gray dashed line: location of the heart; white dashed line: location of the eyes. Anterior up. Scale bars represent 50 μm.
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