ZFIN Image: Dang et al., 2018, Fig. 8

Image

Figure Caption

Fig. 8

Nrp1b and robo2, but not nrp1a, are required for axonal targeting to the DZ.

Tg(BACor111-7:IRES:GAL4; UAS:citrine) (‘or111-7’) expressing OSNs project axons to the CZ in wild-type olfactory bulbs (A, D, G) while Tg(BACor130-1:IRES:GAL4; UAS:citrine) (‘or130-1’) expressing OSNs project axons to the DZ in wild type olfactory bulbs at 72 hpf (J, M, P). In nrp1a-/- larvae, or111-7 axons misproject to the DZ (B, C). Mistargeting of or111-7 axons is not observed in nrp1b-/- (E,F) or robo2-/- larvae. (H, I). However, in nrp1b-/- larvae, or130-1 axons misproject to the CZ (N, O). Similarly, robo2-/- larvae show misprojections of or130-1 axons to the MG (Q, R). Mistargeting of or130-1 axons is not observed in nrp1a-/- larvae (K,L). Fisher’s exact test (two-tailed) * = p<0.05, ** = p< = 0.005. CZ: central zone, DZ: dorsal zone, MG: medial glomerulus, LG2: lateral glomerulus 2, LG3: lateral glomerulus 3. OE: olfactory epithelium, OB: olfactory bulb.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.