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Fig. S2
nkx genes regulate shox2
(A-E) Ventral view, anterior to the top, at 52 hpf illustrates in situ hybridization for shox2. Expression of shox2 is restricted to the IFT in wild-type (black arrow) (n = 11/13) (A) and nkx2.5+/+;nkx2.7-/- (n = 5/6) (B) embryos. However, its expression is expanded throughout the cardiac chambers in nkx2.5-/-;nkx2.7+/+ (n = 9/10) (C), nkx2.5- /-;nkx2.7+/- (n = 12/13) (D), and nkx2.5-/-;nkx2.7-/- (n = 5/7) (E) embryos.
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