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Fig. S4
sgRNA injection does not affect parachordal chain (PAC) development. (A) In 50 hpf Tg(fli1a:egfp) embryos injected with 1nl of 12–15 ng/μl of sgRNA1 failure of PAC development (red asterisk) is observed in fewer than 5 hemisegments at the yolk extension per embryo (48/51). (B) A third of embryos injected with sgRNA1 and Cas9 mRNA have perturbed development of the PAC in 5 or more hemisegments at the level of the yolk extension (16/47). A, anterior; D, dorsal; hpf, hours post fertilisation.
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