ZFIN Image: Heap et al., 2018, Fig. 2

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Figure Caption

Fig. 2

Gal4-positive hypothalamic neurons project axons to the tectal neuropil. (A) Maximum projection of a 6 dpf Gal4^s1113t;UAS:Kaede larva in which expression is strongest in a small number of neurons in the ventral diencephalon, located in the RH (arrow). (B) shows a closeup of the box in (A). Neurites are evident in the tectal neuropil (arrowhead) suggesting that hypothalamic projections may be targeting the tectum. (C) A coronal rotation through an animal with the genotype Gal4^s1113t;UAS:Kaede. The location of labeled cell bodies is indicated with an arrow, and projections to the tectal neuropil are labeled with an arrowhead. (D,E) Images from a Gal4^s1113t;UAS:Kaede, UAS:syn-GFP showing the cell bodies of the RH neurons with photoconverted red Kaede (arrowhead, D) and the green presynaptic terminals of their axons in the tectal neuropil (arrow, E). These channels are merged in (F). Scale bars equal 100 μm.

Acknowledgments

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