ZFIN Image: Sánchez-Iranzo et al., 2018, Fig. S3

Image

Figure Caption

Fig. S3

The transgenic tbx5a:GFP reporter line recapitulates the expression of endogenous tbx5a. a,b Sagittal section through an adult tbx5a:GFP zebrafish heart immunostained for GFP and Myosin Heavy Chain (MHC), (n=8/8). c,d mRNA in situ hybridization (ISH) with a tbx5a antisense riboprobe on the adjacent section shown in a and b. Note expression of GFP and tbx5a mRNA in the trabecular myocardium and their absence of expression in the cortical myocardium (n=8/8). b and d are zoomed views of boxed areas in a and c. e,f tbx5a ISH on a sagittal heart section of an AB wildtype adult zebrafish (n=5). g,h GFP ISH on a sagittal heart section of tbx5a:GFP zebrafish (n=4). at, atrium; ba, bulbus arteriosus; cort, cortical layer; trab, trabecular layer; wt, wildtype. Scale bars, 100 μm

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.