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Fig. 5
Comparison of metastasis in ache wild-type and mutant larvae. Representative metastatic larva images from Hep3B (a) and SKHep1 (b) injections into ache+/? (n = 154) larvae are shown. Green fluorescent dye DiO labeled migrated cells can be seen both in Hep3B (a’) and SKHep1 (b’) cell injections. Injection of Hep3B (c) and SKHep1 (d) into yolk sac of ache−/− (n = 51) larvae did not show a metastatic phenotype (c’,d’) (when there were more than 5 cells in tail region that embryo was counted as positive for metastasis, see (d’) which was counted as negative for metastasis). (e) Positive metastasis percent in ache wild-type and mutant groups were compared where there were significantly more metastatic larvae in ache wild-type group (P = 0.0002, chi-square test). (f) To see the effect of metastasis on local tumor size, tumor sizes of metastasis positive and negative groups were compared. Metastasis negative larvae (n = 129) showed a trend for larger tumors although non-significant (P = 0.1112, T-test) when compared to metastasis positive larvae (n = 60). n.s = not significant.