ZFIN Image: Uribe et al., 2017, Fig. 9

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Figure Caption

Fig. 9

Ectopic expression of meis3 in the neural crest partially rescues gut colonization following RA inhibition. (A) Cartoon schematic summarizing injection and treatment experiments. (B-C) Live images of phox2bb:Kaede/mCherry⁺ 24 hpf embryos injected with (B) pDest-sox10:turq-P2A-mcherry-pA2, or (C) pDest-sox10:meis3-P2A-mcherry-pA2. Lateral views, ot-otic. (D-I) Maximum intensity confocal projections following immunochemistry against mCherry (red) and Hu (cyan) at 75 hpf in larvae expressing (D, F, H) pDest-sox10:turq-P2A-mcherry-pA2, or (E, G, I) pDest-sox10:meis3-P2A-mcherry-pA2, following treatment with DMSO or DEAB, respectively. (H-I) Zoomed in view of the insets from F and G, respectively. Scale bars: 60 μM.

Acknowledgments

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Reprinted from Developmental Biology, 433(1), Uribe, R.A., Hong, S.S., Bronner, M.E., Retinoic acid temporally orchestrates colonization of the gut by vagal neural crest cells, 17-32, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.