ZFIN Image: Vierstraete et al., 2017, Fig. 5

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Figure Caption

Fig. 5

Results of morpholino experiment. (a) Digital gel of RT-PCR (obtained by Labchip®GX software) of uninjected (MO−) and injected (MO+) embryos. Injecting the morpholino results in retention of intron 7, resulting in a transcript containing a premature stop codon. This is visualised as a longer RT-PCR fragment, using primers located in exon 4 and 8 (expected size: 250 bp; in case of intron 7 retention: 1600 bp). In the morphants, there is a clear band of 1600bp, indicative for intron retention. (b) Quantification of Rad51 foci in three morphants (MO+) and uninjected (MO−) embryos. Almost no Rad51 foci are present in the morphants. Error bars display 95% CI. (c) γH2AX foci are present in both irradiated wild types (upper left) and morphants (lower left). Wild types display clear Rad51 foci (upper right), while morphants display no Rad51 foci.

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Acknowledgments

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