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Fig. 4
Growth of testis in 72 hpf embryos grafted into germ cell-depleted and castrated rag1 mutants after 2 months. (a) Histology of testes of 72 hpf embryos grafted into normal, germ cell-depleted, and castrated rag1 mutants. Dotted lines represent testes of the grafts. Scale bar, 100 µm. (b) Comparison of testis size between 2 mpf normal testes and grafts into normal, germ cell-depleted and castrated rag1 mutants. Testes volume was calculated from graft serial sections (n = 9 normal, n = 7 germ cell-depleted and n = 5 castrated) and of 2 mpf males (n = 3). Error bars indicate the standard error. *p < 0.01; **P < 0.05 (paired t-test).