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Fig. 1
Generation and characterization of zebrafish transgenic ATF4 lines (ATs). (A) The transgenic construct, pβ-Act-Tetoff-ATF4-2A-mCherry. (B) Immunoblot analysis of ATF4 expression in AT1 and AT2 fish. Control: wild type (WT). (C) AT1 and WT without Dox treatments (−Dox, panels 1 and 3, 40X magnification, scale bars: 200 μm), and AT1 and WT with (+Dox, panels 2 and 4, 40X magnification, scale bars: 200 μm).
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