ZFIN Image: Shi et al., 2017, Fig. 2

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Figure Caption

Fig. 2

Rbpr2 mRNA expression patterns during zebrafish development analyzed by whole mount In-situ Hybridization (WISH). (A) At the 8-somite stage, zygotic rbpr2 mRNA (purple stain) is expressed in the yolk syncytium and in the mesendodermal cells. Staining is also detectable in the anterior somites (arrow). (B) At 3 days post fertilization (dpf), staining for rbpr2 mRNA expression (purple stain) becomes restricted and is observed in the developing liver (Liv) and Gut (Gut). At the 4 (C) and 5.5 dpf (D) larval stages, rbpr2 mRNA expression is maintained within the liver and intestine. (B–D) Interestingly, unlike zebrafish stra6 mRNA expression in the eye²⁵, rbpr2 mRNA expression was not observed in the developing eyes. (E) Transverse sections through 5.5 dpf zebrafish larvae at three different regions, corresponding to panels F–H. (F–H) Histological analysis of transverse sections reveals rbpr2 mRNA expression (blue) in the liver hepatocytes (Liv) and intestinal enterocytes (Int). Pan, pancreas; PD, pancreatic duct; Liv, liver; Int, intestine. Scale bar = 100 μm (F–H).

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Acknowledgments

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