ZFIN Image: Costa et al., 2018, Fig. 1

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Fig. 1 Deficient dietary phosphorus intake impairs zebrafish (Danio rerio) skeletal development. Vertebral and spinal anomalies are shown in zebrafish fed with T1–T6 levels of phosphorus in the diet (T1 = 0.35%; T2 = 0.65%; T3 = 0.95%; T4 = 1.25%; T5 = 1.55%; T6 = 1.85%). (A) Partial fusion in neural and hemal spines in T2 fishes; (B) Absence of neural and hemal spine fusions in T1 fishes; (C) Absence of parallelism between neural and hemal spines in T2 fishes; (D–F) Caudal vertebral and spinal anomalies; (D) Normal vertebral column in T5 fishes; (E) Deformed vertebral column at the caudal fin region (the dotted line represents the estimated normality) in T1 fishes; and (F) anchylosis in T1 fishes. N and H arrows point to the neural and hemal spines, respectively. Scale bar: 500 μm.

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Acknowledgments

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