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Fig. s3 Ciliary localization analysis of Rhoa in Itga8/Pcdh15a MOs and mutants. A-J: Confocal images from 3dpf (A-G) and 5dpf (H-J) larvae showing Rhoa immunostaining (red) at the apical aspect of neuromast hair cells. Scale: A-G 5µm, H-J: 5.5µm. K-L: Quantification of Rhoa fluorescent intensity at the apical aspect of hair cells. At least 3 independent experiments were performed for each treatment and the total fluorescence intensity expressed as a percentage from control (mean±SEM). Oneway ANOVA followed by Dunnett’s multiple comparisons test or two-tailed Student’s t test ***p<0.001, **p<0.01, *p<0.05. ns=not significant.