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Fig. 3
Figure 3. Chronic high caloric intake inhibits adult neurogenesis and expression of cell cycle regulators in brain tissue of 1-year old zebrafish. (A) Left: schematics showing cerebellar sections with boxes identifying areas presented in the images along with schematic of zebrafish brain identifying coronal planes at which the brain was cut; Right: representative images of the total number of 5-Ethynyl-2′-deoxyuridine (EdU) positive cells (S-phase), green, in Control and HCI fish at ZT11, following 2-h EdU exposure, intraperitoneal (i.p.) injection, Scale: 180 μm. (B) Percent cells in S-phase in 3-year old Control (white bar) and 1-year old HCI (black bar), relative to mean levels in 1-year old Control (100%, white bar); * p < 0.05 vs. 1-year old Control and # p < 0.05 vs. 3-year old Control; (C–E) Daily patterns of mRNA abundance for cyclins A2 and B2, and p20 in 1-year old Control (solid line) and HCI fish (dashed line); n = 5–6 fish per each data point; mean ± SEM, * p < 0.05 HCI vs. Control at specific time points. Grey background—night, 14:10 LD cycle. Zeitgeber time of sample collection, ZT0 = lights-on time.
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