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Fig. s6 Supplementary figure 6. Specification of intrahepatic duct lineage in the absence of the vasculature. (a-b) Whole organ immunofluorescent analysis of 76 hpf wild type (a) and npas4l mutant (b) livers labelled for Prox1 (red), Hnf4a (blue), and Alcama (green). Green Alcama channel is removed in panels below a and b. Intrahepatic duct cells (arrows), expressing both Prox1 and high Alcama, but lacking Hnf4a, are present in both wild type and mutant livers. Scale bars 20μM
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Full text @ Nat. Commun.