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Fig. 3
A. PJA1 mRNA expression was determined by whole-mount in situ hybridization in developing embryos. PJA1 mRNA is expressed in the yolk and ventral side of the embryo at the 13 somite stage (i) and in intestinal and vascular tissues at 28 hour post-fertilization (hpf) (ii). B. Developmental defects were observed in zebrafish embryos injected with 4ng or 8ng of antisense PJA1 morpholino oligonucleotides (MO) but not in embryos injected with same dose of control MO. C. Apoptosis was visualized by acridine orange staining in live embryos, where apoptotic cells are indicated by green fluorescence. Compared to that of the control MO injected embryos at 24 hpf. D. injection of PJA1 MOs severely disrupted embryogenesis (v) and resulted in high levels of apoptosis (vi). Red squares indicate the embryonic brain of zebrafish.
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