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Fig. S19
A gradient of Urocortin3 expression along the A/P axis of primary islets at 25 dpf. A single confocal plane of a primary islet from wild-type animals stained using antibodies against insulin and mouse-Urocortin3 (polyclonal) at 25 dpf. The islet is aligned along A/P axis (anterior is to the top). Beta-cells in the posterior region of the islet exhibit positivity for both insulin (green) and Urocortin3 immunofluorescence (red) (arrows). In contrast, the insulin-positive cells in the anterior region are Urocortin3-negative (arrowhead). Scale bars, 20 μm.
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Full text @ Nat. Commun.