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Fig. S1
The expression of HA-DsRed was induced by Dox treatment in the transgenic embryonic and adult fish harboring the modified pIDM:HA-DsRed transgene. (A) Transgenic zebrafish were generated as described in Figure 1B. Transgenic embryos were treated with Dox at 12 hpf. The treated and untreated embryos at 2 dpf were fixed with 4% PFA for 1 hour and then subjected to cryo-sections. The photo was taken with a fluorescent microscope. (B) Three-month-old transgenic fish were treated with Dox. The picture was taken under a confocal microscope at 15 dpt. Tg+/-, heterozygous transgenic fish; Ctr, untreated control.
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