ZFIN Image: Matsuoka et al., 2017, Fig. S5

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Figure Caption

Fig. S5

VTA formation in vegfaa^bns1, vegfab^bns92, and vegfc^hu6410 mutants. (A) Predicted domain structure of WT Vegfab isoforms -171, -210, and Vegfab^bns92. Vegfab-171 and -210 consist of a signal peptide (SP), a knot motif, and heparin (Hep) and/or Hep/Nrp1 (Nrp) binding domains. vegfab^bns92 encodes a truncated peptide (p.Met88Trpfs*18). (B and C) 8 dpf Tg(hsp70l:vegfaa₁₆₅) vegfaa^−/− (B) and vegfc^−/− (C) larval trunk vasculature visualized by Tg(kdrl:EGFP) expression. Tg(hsp70l:vegfaa₁₆₅) vegfaa^−/− embryos were heat-shocked at 10 and 24 hpf to rescue their early vascular phenotype. VTAs are present in all of the vegfaa^−/− (B; n = 11) and vegfc^−/− (C; n = 10) larvae examined. (D and E) 8 dpf vegfab^+/+ (D) and vegfab^−/− (E) larval trunk vasculature visualized by FITC-dextran microangiography. vegfab^−/− larvae lack lumenized VTAs (yellow arrows, E).

Acknowledgments

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