ZFIN Image: Shen et al., 2017, Fig. S4

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Figure Caption

Fig. S4

Morphological analysis of adult heterozygotic and embryonic homozygotic Ikk2 mutants obtained from heterozygotic parents. (A-D) Adult ikk2^-/- fish with skeletal defects. Adult ikk2^-/- fish are short with curved skeleton (B, D) as compared to wild types (A, C). (E, F) Adult ikk2^-/- fish are prone to skin lesion accompanied by loss of scales and hypopigmentation. Scale bar in A-D: 1cm; scale bar in E,F: 0.1 cm. (G-N) In MZikk2^-/- escapers cells detach in embryonic shield (arrow) at late gastrula. (G, G’), later stage embryos contain increased cell debris under the chorion (I, arrowed) and show defects in posterior body (K-N). G, lateral view. G’, dorsal view. J-N, lateral view, 24 hpf. K-N, ikk2^-/- embryos with defects in posterior body are classified into four categories (I to IV) based on severity of the notochord defects. This phenotype mimics that of zygotic ikk2^-/- mutants (Figure S2, A-E). Scale bar in G-N: 500 μm.

Acknowledgments

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