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Fig. 3
Metformin suppresses pronephric epithelial proliferation in pkd2 morphants. (A) Representative confocal immunofluorescence images showing proliferative cells (arrows) in pkd2 morphants with and without metformin (10 mM) treatment. Embryos were stained using anti-PH3 (red) to mark proliferating cells and anti-α6 F (green) to label the pronephric ducts at 48 hpf. (B) Counts of anti-PH3- and anti-α6F-positive cells in the anterior and posterior pronephric ducts (n = 14–17 per group). *P < 0.05, ***P < 0.001. Data represent two independent experiments. Scale bar, 20 µm.
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