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Fig. S1
Expression patterns and immunoreactivity of recombinant mCherry, mCherry-Cldn5a and mCherry-cldn5b.
(A—C) Expression patterns of recombinant proteins in 6 hpf embryo. Representative images of recombinant mCherry (A), mCherry-Cldn5a (B) and mCherry-Cldn5b (C). (A’–C’) Immunoreactivity to anti-mammal Cldn5 antibody of recombinant proteins. Representative images of recombinant mCherry (A’), mCherry-Cldn5a (B’) and mCherry-Cldn5b (C’). Scale bar: 20 μm.
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