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Fig. S4
Gross morphology and heart laterality defects of cldn5a MO1 and MO2 injected embryos.
(A—C) Gross morphology of control morphants (A), cldn5a MO1 injected embryo (B), and cldn5a MO2 injected embryo (C). (D—F) Visualization of heart by in situ hybridization of cmlc1 in 30 hpf embryos. Representative images of control morphants (D), cldn5a MO1 injected embryo (E), and cldn5a MO2 injected embryo (F). (E) Statistical stacked bar graph (blue; normal, orange; middle, grey; reversed, control morphants; n = 68, cldn5a MO1 injected embryos; n = 55, and cldn5a MO2 injected embryos; n = 53).
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