|
Fig. S2
Generation of a new allele of the gosh mutant using the CRISPR/Cas9 system
(a) Sequence of exon1 of the aipl1b gene in embryos showing no OKR response. A 17-bp insertion causes a frame-shift of the coding region, which subsequently induces a premature stop codon. (b) Diagram showing the strategy used to confirm that gosh mutant gene encodes aipl1b. (c) Labeling of retinas of 7-dpf no-OKR zebrafish embryos with anti-blue opsin and zpr1 antibodies or anti-rhodopsin and zpr1 antibodies. Photoreceptors are abnormal in shape, and blue opsin is mislocalized to the plasma membrane of the cell body (arrows). However, rhodopsin is normally localized at the OS.