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Fig. 6
Impairment of rim involution leads to mispositioning of neuroepithelial cells.
(A) Time-lapse imaging of control and ezrin morphant condition in Tg(vsx2::GFP, βactin::mKate2ras) background. Arrowheads mark rim zone in control. Arrows mark rim cells that failed to migrate in ezrin morphant. Frames from Videos 19 and 20. Time in h:min. Videos started at 16 ss -17 ss. N = 9. (B) Confocal scans of optic cups at 30 ss in control, laminin morphant, Opo morphant and ezrin morphant conditions in Tg(vsx2::GFP, βactin::mKate2-ras). Embryos were stained for GFP and mKate-2. Arrows point at the ectopic RNE cells. N = 7 each. (C) Brightfield image of 30 ss ezrin morphant showing secondary optic cup phenotype. Arrow marks secondary invagination zone. (D) Brightfield images of 36 hpf control embryos, ezrin morphants and opo morphant. Scale bars in (A,B) =10 µm and (C,D) = 50 µm.