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Fig. 6
Knockout of fads2 increases retinalapoptosis in a larval zebrafish model of light-induced retinopathy. (A) Protocol for light-induced retinal damage in larval zebrafish. Zebrafish were shielded from light between 3 and 5 days post-fertilization (dpf) and then exposed to normal conditions (14 h 250 lux/10 h dark) or intense light (13,000 lux) for 48 h at 27 °C. After light exposure, whole-mount TUNEL staining was performed. (B) Representative images of TUNEL staining in the retina of control or fads2 knockout zebrafish exposed to intense light (indicated as light +) or normal light conditions (light −). Scale bars, 100 μm. (C) Quantitative analysis of retinal apoptosis in zebrafish exposed to the conditions shown in (B). *p < 0.05, ****p < 0.0001. Data are the mean ± SEM of 11–14 eyes/group.