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Fig. S5 Expression of pnoca and scinlb genes in alpha pancreatic cells of zebrafish embryos. Co-labeling by in situ hybridization at 30 hpf of new discovered genes with cell type-specific markers for alpha and beta cells (n > 10). A and B. pnoca is expressed in alpha cells (A, arrows) while no expression was detected in beta cells (B). scinlb was detected specifically in alpha cells (C, arrows) but not detected in beta cells (D). gcga: glucagon a, ins: insulin, Z: no endocrine gene with no conserved expression, N-orth: Endocrine-enriched zebrafish gene with no described ortholog in human and/or mouse.