ZFIN Image: Pimtong et al., 2014, Fig. S6

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Figure Caption

Fig. S6

Enforced expression of drl is not sufficient to rescue drl.3 morphants.

(a) WISH of drl, drl.1, drl.2, and drl.3 in 24 hpf L3MM- and L3MO-injected embryos. Lateral views, head to the left (right) and dorsal anterior views (left). Number of embryos with representative phenotypes per total embryos analyzed is indicated. (b) Percent of control (L3MM) or drl.3 morphants (L3MO) ± drl mRNA that display normal (wt) or low (estimated s 60% of normal) numbers of o-dianisidine⁺ cells. Two amounts of drl mRNA were tested: 3 ng/embryo and 6 ng/embryo. Co-injection of L3MO + 3 ng of drl mRNA per embryo resulted in 32% of embryos showing decreased numbers of o-dianisidine⁺ cells versus 34% of drl.3 morphants. Similarly, 6 ng drl mRNA + L3MO resulted in 32% of the embryos displaying decreased erythrocytes compared to 29% of the L3MO-injected morphants. P values determined by Fisher's exact test.

Acknowledgments

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