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Fig. S8
Stage-dependent dorsalisation following Vrtn overexpression. (A) An uninjected embryo at 24 hpf. (B-D) Different degrees of dorsalised embryos, which can be categorised into C5 (B), C3 (C) and C2 (D), respectively. (E) Stage-dependent decline of the dorsalising effect following Vrtn overexpression. Notice the weak dorsalising activity when vrtn mRNA is injected after first cleavage stage. Numbers on the top indicate total embryos scored from two independent injection experiments. Scale bar: 250 μm.
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