ZFIN Image: Shao et al., 2017, Fig. S5

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Figure Caption

Fig. S5

Knockdown of bmp2b in the margin mimics the dorsalising effect of VAb@2cell. (A) Diagram showing the coinjection of bmp2bMO (2 ng/embryo) and RLDx in the yolk cell at 128-cell stage. (B,C) RLDx labelling in the margin of a sphere stage embryo with lateral (B) and animal pole (C) views. White dots outline the embryo. (D) Representative phenotypes at 12 hpf and 24 hpf resulted from bmp2b knockdown at indicated stages. The distribution of bmp2bMO is only restricted in YSL following injection in the yolk cell at 512-cell stage. (E) Comparison of the efficiency of dorsalisation following bmp2b knockdown at 128-cell and 521-cell stages. Numbers on the top indicate total embryos scored from two independent experiments. Scale bars: 250 μm.

Acknowledgments

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