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Fig. 5
Prostaglandin signaling influences nephron patterning after gastrulation.
Embryos were treated with 0.3% DMSO or 1% DMSO vehicle control, (A) 30 μM indomethacin, or (B) 100 μM dmPGE2 from the 12 ss to the 28 ss and stained for the PST (trpm7), DE (slc12a1), and DL (slc12a3) (purple) using WISH at the 28 ss stage. Black bars indicate segment gene expression domain. Red scale bar, 70 µm. (C) The PST, DE, and DL domains were measured in microns (n = 5 for each control and experimental group). Data are represented as ± SD, ANOVA used to compare samples, *p<0.01, **p<0.05, where n.s. indicates not significant.
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