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Fig. S4
Measurement of heart fluorescence over time for the pronephric functional assesment in zebrafish.
Texas Red® tagged 70 kDa dextran was injected into the heart of ELMO1 mosaic mutants, PDX1 morphants and their respective controls, in zebrafish embryos, aged 72 hpf. Images were taken at 1 hpi, 24 hpi and 48 hpi and the relative fluorescence in the heart region, highlighted in red, was measured using imageJ. A. indicates the development and loss of fluorescence in an ELMO1 mosaic mutant over indicated time. The white line represents a scale bar of 200 μm.
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