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Fig. 4
Establishment of Tg[epdr1-hs:eGFP].
(a,b) A heterozygous Tg[epdr1-hs:eGFP] embryo. eGFP expression was detected in the telencephalon (tc), the midbrain-hindbrain boundary (MHB), the hindbrain (hb) and spinal neurons (sp) at 30 hpf. The epdr1 expression in the optic stalk (os) was detected by confocal microscope (b). Autofluorescence of epidermis was detected (*). (c,d) Expression of epdr1 in the tc, the os, the MHB, the hb and the sp at 24 hpf. The expression of epdr1 was examined by whole-mount in situ hybridization using an anti-sense epdr1 RNA probe.
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