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Fig. 4
Expression patterns of ion transporters and sodium accumulation in gills of prl-deficient larvae.
(A–F) Whole mount in situ hybridization assay of solute carrier family 12, member 10, tandem duplicate 2 (slc12a10.2) and ATPase, Na/K transporting, α 1a polypeptide, tandem duplicate 5 (atp1a1a.5) expression in gills of wild-type larvae (A,D), prl-deficient larvae (B,E) and prl -deficient larvae injected with prl mRNA (C,F) at 5 dpf in regular zebrafish egg water. (G) counts of atp1a1a.5 and slc12a10.2 expression cells in gills based on in situ results. (H) Expression levels of atp1a1a.5 and slc12a10.2 in head tissue from wild-type, prl-deficient larvae and prl-deficient larvae injected with prl mRNA at 5 dpf in regular zebrafish egg water via qRT-PCR assay. (I,J) Sodium green staining in gills of living larvae of wild-type (I) and prl-deficient larvae (J) at 6 dpf in regular zebrafish egg water (dorsal views). (a,b) different letters in two group mean significant difference (P < 0.05). The qRT-PCR result shown here is the representative of the results obtained in two separate experiments. For in situ hybridization results, at least 12 embryos/genotype were analyzed in two separated experiments. For cell counts, 8 embryos were analyzed in two groups (N = 8).