ZFIN Image: Demy et al., 2017, Fig. S2

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Figure Caption

Fig. S2

Macrophages do not colonize the CNS in mon^TB222 mutants and Trim33 morphants. (A-B) Csfr1a ISH at 36 hpf shows that the initial primitive macrophage production and spreading in the mon^TB22 mutant (B) are similar to the sibling (A). (C-D') In vivo images of retinas (C,D) or midbrain (C'-D') of 3 days old Tg(HuC:GFP; mpeg1:Gal4; UAS:NfsB-mCherry) embryos injected (D-D') or not (C,C') with the Trim33 morpholino. Trim33 KO (D,D') causes macrophage depletion in both CNS compartments. (E-G) Lplastin whole-mount immunodetection (combined with DAPI detection of cell nuclei) in mon^TB222/Tü siblings (E,E') and mutants (F,F') reveals numerous leukocytes (macrophages) in the retinas (E) and midbrain (E') of 3 days old siblings, whereas there are only a few in the mutants. Counting of these cells every 6 hrs from 30 to 66 hpf reveals that mon^TB222 mutants have less leukocytes in the CNS at all stages. Ctrl, control; Mo, morphant; Mut, mutant; nb, number; Sib, sibling. **, P value <0.01; ***, P value < 0.001.

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Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.