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Fig. 2
Incubation in high [Ca2+] enhances (a) FGF23 mRNA signals and (b) expression levels in 2 and 4 dpf zebrafish larvae. In situ hybridization was used to detect fgf23 signals, and gene expression was analyzed by qPCR (values were normalized to those of β-actin). Student t test, ***P < 0.001. Values are the mean ± SD (n = 6). Scale bar: 50 µm.
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