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Fig. S1
(A) Western blot detection of Insm1a protein in embryos injected with 6 ng insm1a antisense morpholino or 6 ng control morpholino showing a marked reduction of Insm1a protein in the insm1a morphants. The experiment was repeated two times. (B,C) Fluorescent images of cldnb:lynGFP embryos at 48 hpf injected with control-MO (con MO) (B) and con MO + p53 MO (C). Scale bars: 100 μm. (D) Quantification of the number of neuromasts along the body at 48 hpf in con MO (n = 20) and con MO + p53 MO (n = 20). There was no significant difference (p > 0.05). (E,F) Transmitted light images of 72 hpf control morpholino-injected embryo (con MO) (E) or insm1a morphants (insm1a MO) (F). The overall morphology of the insm1a morphants was indistinguishable from controls. Scale bars: 100 μm.