ZFIN Image: He et al., 2017, Fig. 4

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Fig. 4

Insm1a is required for cell proliferation in the migrating pLLp. (A,B) Representative images of the BrdU immunostaining for proliferating cells in the pLLp in controls (A1–A3) and insm1a morphants (B1–B3) at 32 hpf showing that knocking down of insm1a impairs rosette assembly and cell proliferation. The primordium is outlined with a dotted line. (A3′–B3′) BrdU-positive cells in the rosette at high magnification. White arrows indicate rosettes. Scale bars: 10 μm. (C–E) Quantification of rosette numbers (C), cell numbers (D), and BrdU+ primordium cells (BrdU index) (E) in controls and insm1a morphants. Data are expressed as mean ± s.e.m. ***p < 0.001; **p < 0.01.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Mol. Neurosci.