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Fig. S2
Vascular glut1 expression in WT embryos with and without BNF treatment.
A-C) Overview and high magnification images of whole mount ISH for glut1 in WT embryos at 52 hpf. Vascular expression is limited to the brain capillaries (yellow arrowheads indicate individual CtAs). D-F) Glut1 expression in WT embryos treated with 1 uM BNF. Staining intensity is notably decreased in brain vessels. Scale bar in overview is 500 um, and 100 um in high magnification images. Abbreviations–BNF: beta-naphthoflavone, CtA: central artery, DMSO: dimethylsulfoxide, hpf: hours post fertilization, ISH: in situ hybridization.
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