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Fig. S3 Redundancy of fsta and fstb in hyoid skeletal development. (A) Alcian blue and Alizarin red staining of control, fsta, fstb, and fsta; fstb double mutant embryos at 5 dpf. In fsta but not fstb single mutants, the Sy is shortened (red arrow), and the hyoid joint is fused (black arrowhead). fsta; fstb double mutants display a slight enhancement of craniofacial defects compared to fsta mutants. (B) Quantification of fused hyoid joints and Sy length (number of sides examined for fsta, fstb, and fsta; fstb mutants = 14, 10 and 23, respectively). *p < 0.05 in student’s t-test.
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