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Fig. S7
Method for quantifying Fn intensity in the endocardial population. (A-A', C-C') Single XY confocal Z-plane images were used to quantify the Fn intensity (magenta) in the endocardial populations (green) at 10s. (A', C ') Fn only. The rectangular areas, which span the leading and trailing regions of the endocardial population, were used for plot profile analysis. Yellow dashed line: midline. (B, D) The relative Fn intensity in the endocardial population as determined by plot profile measurement in A' and C'. Scale bars: 10 μm.
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Reprinted from Developmental Biology, 414, Xie, H., Ye, D., Sepich, D., Lin, F., S1pr2/Gα13 signaling regulates the migration of endocardial precursors by controlling endoderm convergence, 228-43, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.