ZFIN Image: Gau et al., 2017, Fig. S7

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Figure Caption

Fig. S7

Loss of runx or cbfb expression did not affect the expression of nociceptive subtype markers.

A-I, Colormetric in situ hybridization for cgrp (A-C), ret (D-F), and scn1α (G-I) in the runx3^w144/w144 and cbfb^w128/w128 mutants focusing on the TG at 24hpf and 3dpf. J-M, Antibody staining of Kaede (red) in cbfb^w128/w128; Isl1SS:Kaede mutants and of GFP (green) in cbfb^w128/w128; P2X3.2:eGFP mutants at 24hpf and 3dpf. N-O, Quantification of Kaede+ and GFP+ cells in the TG at 24hpf and 3dpf. P- S, Colormetric in situ hybridization for scn1α in the runx1^W84X/W84X, runx3^w144/w144 and cbfb^w128/w128 mutants focusing on the RBs at 24hpf and 3dpf. T, Quantification of marker gene expression as %WT in RBs at 24hpf. Arrow, RBs; Arrowhead, TG. Scale bar: A-M 100 μm, N-Q 20μm. Embryos per condition (n = 3–11). All error bars represent S.E.M.

Acknowledgments

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