ZFIN Image: Paksa et al., 2016, Fig. S10

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Figure Caption

Fig. S10

Bilateral positioning of PGC clusters does not require the notochord or blood vessles.

One-color in situ hybridization on wild-type (a-b), clo^m39 (a-d) and flh^tk241 (e-f) embryos. PGCs are stained with nos3 (a-f, arrows on the left-panel images) and endothelial cells with kdrl probe (a-d, arrowheads in a-b). Despite the lack of endothelial cells, PGCs arrive at the target (c, arrow) and form two separate clusters at 28hpf (d; dotted lines in inset) in clo mutant embryos (number of embryos examined: N=12), similar to wild-type embryos (a-b; N=60). In embryos lacking notochord (flh^tk241), PGCs form two bilateral clusters at the gonad region at 24hpf (e-f; dotted lines in inset; N=15). Scale bars 50μm. Lateral (left panels) and dorsal (right panels) views are shown.

Acknowledgments

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