ZFIN Image: Paksa et al., 2016, Fig. S6

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Figure Caption

Fig. S6

Functionality of the lpp MOs

(a) Determination of the efficiency of translation-blocking MOs using EGFP reporters. lpp MOs specifically inhibit the translation of the downstream egfp, as demonstrated by the loss of EGFP signal, while the signal persists in co MO-treated embryos. mcherry is used as an unaffected control. N is the number of embryos assayed. (b) Functionality of lpp1 splice-blocking MO using a PCR-based assay with primers presented in Supplementary Table 8. The mRNA level is largely reduced when employing the lpp1 MO, as compared with odc used as control mRNA that is not affected. DNA marker: GeneRuler 1 kb DNA Ladder.

Acknowledgments

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