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Fig. S3
Reporter assays for putative endothelial enhancers, relating to Figure 5. (A) Proportion of cryaa:mcherry-positive embryos with endothelial Egfp expression following injection with indicated construct. (B) dusp5 locus. Black box indicates element used for reporter assay with preferential mapping of ATAC-Seq reads in GFP-plus, but not GFP-minus nuclei. Grey box indicates fragment of dusp5 promoter used for promoter assays. (C-J) Epifluorescent overlay images of green and red fluorescence in embryos injected with indicated reporter constructs. “Enh-basP” – indicated enhancer upstream of basal promoter; Prom – promoter element from indicated gene; Enh/Prom – composite element with enhancer and cognate promoter from indicated gene. Ratios in left bottom corner denote number of embryos with endothelial green fluorescents over the number of cryaa:mcherry expressing embryos. Scale bar is 250 μm.