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Fig. 7
Regeneration of iridophores and melanophores after ErbB inhibition.
Treatment with PD168393 during embryogenesis (see methods) results in (a–j) gaps of different sizes (1–12 metamers) in the striped pattern of metamorphosing fish. Overall 15 fishes carrying gaps between 3 and 12 metamers were imaged through metamorphosis. Red numbers (a–i) indicate the segments that are devoid of iridophores at the onset of bright-field imaging. Dashed lines indicate vertical and horizontal myosepta. On 24 dpf (g) a spot of regenerating iridophores appears in the middle of the gap and it expands laterally (white arrow in g,h,i). (k) Time (in days) taken to fill the gaps of variable sizes in the stripe pattern. (l) Increase in numbers of melanophores in individual fish (n=5) during regeneration of gap metamers at three different time points, 24, 28 and 34 dpf. Scale bars, a–j: 250 μm.